Abstract: As living coordinates of urban historical culture, ancient and notable trees require improved innovative mechanisms for the transmission of their cultural values. Investigating innovative pathways for activating their cultural values holds significant importance for perpetuating historical-cultural continuity, strengthening ethnic cultural confidence, and advancing cultural industries. This paper examines 8725 ancient trees along Beijing's central axis, systematically analyzing their resource distribution, current cultural value presentation, and transformation requirements. Through empirical case studies and impact assessment, this paper proposes a synergistic innovation pathway of "cultural tourism collaboration+digital technologies". Furthermore, the study explores a cultural-tourism coordination mechanism underpinned by "conservation primacy+format innovation" and the development of a digital cultural system based on "technology empowerment+value decoding." The resulting pathways for cultural value activation and transmission effectively enhance communicative efficacy, intensify conservation advocacy, and provide replicable paradigms for expanding cultural-tourism integration frameworks and perpetuating the cultural lineage of ancient trees along the Central Axis.